Digitalni repozitorijum
Univerziteta u Beogradu
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Visualization of Extracellular Vesicles Derived from Dental Mesenchymal Stem/Stromal Cells Using SEM and Immunogold TEM Analyses
Milivojević, Marija
Janaćković, Djordje
Čolić, Miodrag
Bekić, Marina
Tomić, Sergej
Kosanović, Maja
ABSTRACT Extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have emerged as promising therapeutic agents in regenerative medicine. EVs are nanosized, membrane-limited vesicles containing protein and RNA cargo, released by various cell types for intercellular communication. It has been shown that EVs derived from MSCs play a significant role in the tissue regeneration process [1]. Dental MSCs such as human stem cells from the dental pulp of deciduous teeth (SHED cells) are easily accessible MSCs with high proliferative capacity [2]. The objective of this study was to isolate and characterize EVs derived from SHED, to explore their potential utility in regenerative medicine applications. SHED cells were isolated from deciduous teeth from three donors, and their morphology and osteogenic potential were assessed through optical microscopy and Alizarin Red staining of cells cultured in osteogenic cell media for 21 days.
engleski
2024
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Extracellular vesicles (EVs), mesenchymal stem/stromal cells (MSCs)