Naslov (eng)

Cytotoxicity and ROS scavenging potential of Teucrium montanum L. methanolic extracts in HaCaT cells

Autor

Topalović, Dijana
Pirković, Andrea
Zbiljić, Miloš
Spremo-Potparević, Biljana
Živković, Lada
Jovanović Krivokuća, Milica
Marčetić, Mirjana

Publisher

Opis (eng)

ABSTRACT Teucrium montanum L. (TM) is a perennial species of the Lamiaceae family, traditionally used in folk medicine for its diverse therapeutic properties. Although previous studies have explored the biological activity of TM extracts, their effects on skin cells have not been investigated. This study examined the cytotoxic and antioxidant effects of two methanolic extracts of T. montanum (TM1 and TM2), obtained from serpentinite and limestone, across a concentration range of 12.5–400 μg/mL on human keratinocyte (HaCaT) cells. Both extracts showed a concentration-dependent cytotoxic effect in the MTT assay, with no significant impact on cell survival up to 100 μg/mL. At higher concentrations (200 and 400 μg/mL), cell viability decreased significantly, with TM1 exhibiting greater cytotoxicity than TM2. Antioxidant potential was assessed by the H2DCFDA assay, revealing that neither extract altered basal reactive oxygen species (ROS) levels, but higher concentrations increased ROS formation under H2O2- 42 induced oxidative stress.

Jezik

engleski

Datum

2026

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

eucrium montanum L. / methanolic extracts / MTT assay / H2DCFDA assay / HaCaT cells / metanolni ekstrakti / MTT test / H2 DCFDA test / HaCaT ćelije

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o:25140

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