Naslov (eng)

Caveolin-1 as potential biomarkers of prostate cancer Evidence from Serbian population

Autor

Matijašević Joković, Suzana
Korać, Aleksandra
Kovačević, Sanja
Đorđević, Ana
Filipović, Lidija
Dobrijević, Zorana
Savić-Pavićević, Duška
Vuković, Ivan
Popović, Milica
Brajušković, Goran

Publisher

Opis (eng)

Caveolin-1, a membrane scaffolding protein, plays a key role in regulating cell proliferation, migration, and angiogenesis. Its overexpression in prostate cancer (PCa) cells is significantly associated with increased tumor aggressiveness. The aim of this study was to investigate whether caveolin-1, found in plasma-derived exosomes, could serve as a liquid biopsy biomarker for PCa. Exosomes were isolated from plasma samples of 39 patients with PCa and 33 patients with benign prostatic hyperplasia (BPH), used as the control group, using the Total Exosome Isolation Kit (from plasma) according to the manufacturer’s instructions. Morphological characterization of the isolated exosomes was performed using transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking analysis (NTA), and flow cytometry. Western blot analysis confirmed the presence of the typical exosomal marker CD63, as well as caveolin-1 in plasma exosomes. The results showed no statistically significant difference in exosomal caveolin-1 levels between PCa and BPH patients (p=0.349). However, when PCa patients were stratified according to standard prognostic parameters and tumor aggressiveness scores, the relative protein levels differed between subgroups.

Jezik

engleski

Datum

2025

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-ND 4.0 - Creative Commons Autorstvo - Nekomercijalno - Bez prerada 4.0 International License.

http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode

Predmet

exosomes, liquid biopsy, prostatic hyperplasia

Deo kolekcije (1)

o:25140

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