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Univerziteta u Beogradu
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Comparative Study of Natamycin Encapsulation in Liposomes: Thin-Film vs. Proliposome Methods for Enhanced Stability, Controlled Release, and Efficacy Against Milk Spoilage and Pathogenic Microorganisms
Čutović, Natalija
Batinić, Petar
Marković, Tatjana
Petrovic, Jovana
Obradovic, Milena
Bugarski, Branko
Jovanović, Aleksandra
The aim of this study was to evaluate liposomal particles as a potential delivery system for natamycin, a widely known antimicrobial agent used in the food industry. The goal was to prolong its diffusion into the surrounding medium. Natamycin-loaded liposomes were prepared using two methods (proliposome and thin-film) and two different phospholipid mixtures. The characterization of natamycin-loaded liposomes was performed in terms of their chemical composition (FT-IR analysis), encapsulation efficiency (EE), and antimicrobial potential against spoilage and pathogenic microorganisms that can be found in milk and milk products. During the 60-day storage period, their size, polydispersity index (PDI), and zeta potential were measured. The in vitro release kinetics of natamycin from liposomes were also assessed, and the results showed a significantly lower release rate of the drug when it was encapsulated. EE showed a high level of natamycin encapsulation (>80%), which was confirmed with FT-IR analysis. The stability study indicated that these systems were stable over a 60-day storage period, as the zeta potential of all formulations was ~−25 mV. Satisfactory antimicrobial performance of the developed liposomes against Listeria monocytogenes, Yersinia enterocolitica, Candida tropicalis, Candida parapsilosis, and Aspergillus flavus (MIC values from 0.00625 to 4 mg/mL) indicates that loading of natamycin into liposomal carriers was an adequate method for their encapsulation and delivery in the milk industry.
engleski
2025
Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.
http://creativecommons.org/licenses/by/4.0/legalcode
antifungal agent; food industry; encapsulation; stability
10.3390/foods14173064