Naslov (eng)

Assessment of X Chromosome Centromere Instability in Alzheimer’s Disease: A Quantitative FISH Approach

Autor

Spremo-Potparević, Biljana
Popović, Petar
Topalović, Dijana
Pirković, Andrea
Perry, George
Živković, Lada

Publisher

Opis (eng)

ABSTRACT Chromosomal instability in Alzheimer’s disease (AD) neurons has been previously reported. This pilot study aimed to establish a quantitative technique for assessing X chromosome centromere signals using fluorescence in situ hybridization (FISH). Hippocampal brain tissue was collected at autopsy from sporadic AD patients and age- and gender-matched controls. FISH was utilized to detect and measure the intensity of hybridization signals for X chromosome centromeres in the interphase nuclei of hippocampal brain cells. The premature centromere division (PCD) phenomenon, marked by a close bipartite signal appearing as two separated FISH spots, was examined to see if the hybridized DNA amount in each spot matched the expected centromere DNA amount.

Jezik

engleski

Datum

2025

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Alzheimer’s disease; premature centromere division; X chromosome; chromosomal instability; fluorescence in situ hybridization

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP