Application of Lion's Mane Mushroom in Cosmetics: The Influence of Drying Method and Solvent
Malović, Anja
Nikolić, Joka
Jovanović, Aleksandra
Pjanovic, Rada
Banjanac, Katarina
Veljković, Milica
Djordjevic, Verica
Petrovic, Predrag
Introduction: Mushrooms have traditionally been studied for their nutritional and medicinal properties; however, growing interest has recently emerged in their potential applications in cosmetics. Increasing evidence suggests that mushrooms are a rich source of valuable cosmeceuticals, particularly compounds with anti-aging properties, which are among the most sought-after in skincare. As the market for mushroom-based cosmetics continues to expand, there is a clear need for further scientific research in this area. Lion’s mane mushroom (Hericium erinaceus) is a fungi species that parasites on oaks and produces characteristic irpicoid fruiting bodies; in recent years, it has gained popularity as a commercially cultivated edible species with potential health benefits. The aim of this study was to investigate the potential cosmetic benefits of lion’s mane mushroom. Experimental: The effect of drying was assessed using two methods: heat drying (38°C, 24 h) and freeze-drying (-40°C, 0.12 mbar, 24 h). Additionally, the influence of solvent polarity was evaluated using water and water-ethanol mixtures containing 30%, 50%, and 70% ethanol. The resulting extracts were subjected to chemical and biological characterization, with a focus on compounds and activities beneficial for skincare. Total carbohydrates were quantified via the phenol-sulfuric acid method. Sugars and polyols were analyzed using HPLC, and glucan content was determined with a Megazyme® β-Glucan Assay Kit. Antioxidant activity was assessed in vitro using ABTS, CuPRAC, and β-carotene bleaching assays. Antityrosinase activity was evaluated via an in vitro enzymatic assay.
engleski
2025
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