Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility
White, Ruby
Sotillo, Javier
Ancarola, María Eugenia
Borup, Anne
Toftegaard Boysen, Anders
Brindley, Paul J.
Buzás, Edit I.
Cavallero, Serena
, et al.
Kosanović, Maja
ABSTRACT Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms.
engleski
2023
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Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.
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electron microscopy, EV guidelines, EV reporting, extracellular vesicles, helminths, parasites