Naslov (eng)

Immunomodulatory Potential of Trophoblast-Released Extracellular Vesicles on Activated PBMCs

Autor

Jovanović-Krivokuća, Milica
Kosanović, Maja
Pirković, Andrea
Bojić-Trbojević, Žanka
Nacka-Aleksić, Mirjana
Vilotić, Aleksandra

Publisher

Opis (eng)

ABSTRACT During pregnancy, trophoblasts express and secrete a battery of immunomodulatory molecules to sustain immune tolerance at the feto-maternal interface. The study aimed to investigate the effects of extracellular vesicles (EVs) released by first trimester extravillous trophoblast HTR-8/SVneo cell line on activated peripheral blood mononuclear cells (PBMCs), and their ability to modulate cellular proliferation and the expression of inflammatory molecules. EVs were isolated from HTR-8/SVneo cell-conditioned medium and characterized for size, quantity, and exosomal markers using nanoparticle tracking analysis (NTA) and Western blot, respectively. Subsequently, PBMCs were isolated from healthy human donors and activated by PHA.

Jezik

engleski

Datum

2023

Licenca

© All rights reserved

Predmet

extracellular vesicles, activated peripheral blood mononuclear cells (PBMCs)

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP