Naslov (eng)

Three methods of isolating EVs from pleural effusion samples of patients with advanced lung adenocarcinoma-potential applications in clinical practice?

Autor

Vuković, Miodrag
Filipović, Lidija
Petrović, Nina
Zečević, Andrej
Kosanović, Maja
Tanić, Miljana
Janković, Radmila
Stanojković, Tatjana
Popović, Milica
Korać, Aleksandra
Čavić, Milena

Publisher

Opis (eng)

ABSTRACT Introduction: Pleural effusion (PE) occurs in 17-23% of lung cancer (LCa) patients and it contains extracellular vesicles (EVs) from cancer cells, representing “liquid biopsy” of LCa. Isolated PE-EVs could be used for LCa diagnosis or monitoring its progression/therapy. However, there is still no standard method for isolation of EVs from pleural fluid. The aim of this work is to compare PE-EVs isolation methods that could be employed both in research or clinical settings. Methods: PE samples diluted in PBS (1:1) from patients with advanced non-small cell lung cancer (NSCLC) were utilized. Three methods for isolating EVs were employed: an in-house spherical porous methacrylate-based copolymer coupled with VHH antibodies (chromatography method-CH), ultracentrifugation (UC), and the Norgen Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Commercial kit-CK). For each EVs isolation method, efficiency was monitored in terms of the amount of starting sample, time required for vesicle isolation, yield, quality of the obtained isolates and overall cost. Results: In terms of the amount of starting sample, the CH and CK have an advantage, allowing work with as little as 500μL, whereas UC required several milliliters of sample (in our case, due to the rotor type, 12mL minimum).

Jezik

engleski

Datum

2024

Licenca

© All rights reserved

Predmet

Pleural effusion, extracellular vesicles,

Deo kolekcije (1)

o:25140

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