Digitalni repozitorijum
Univerziteta u Beogradu
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Outer membrane vesicles of plant beneficial bacterial strain Paraburkholderia phytofirmans PsJN make a contact with Arabidopsis thaliana rooots
Nešić, Sofija
Divac Rankov, Aleksandra
Spasovski, Vesna
Kosanović, Maja
Nikolić, Dragana
ABSTRACT Extracellular vesicles (EVs) are recognized as important mediators of intercellular communication in both eukaryotes and prokaryotes. These lipid membrane-coated spherical nanoparticles carry proteins, nucleic acids and other cellular products, and facilitate exchange of these biomolecules among cells within an organism, but also between cells of different organisms, belonging to different species and even kingdoms. Outer membrane vesicles (OMVs), EVs produced by Gram-negative bacteria, are a significant mediator of microbial communication, involved in biofilm formation, virulence, and modulation of host immunity. OMVs of both pathogenic and plant beneficial bacteria have been shown to elicit plant immune responses. Investigations on the modes of OMV-plant cells interactions are still in their infancy, but gain rising attention. Aiming to monitor the interaction between OMVs of Paraburkholderia phytofirmans PsJN, a plant growth promoting bacteria, and Arabidopsis thaliana roots, we isolated OMVs from bacterial culture in mineral medium, using an ion-exchange chromatography system. Isolated OMVs were labeled with lipid binding fluorescent dye Vybrant™ DiD and unbound dye was removed by washing vesicles on ultrafiltration columns. The same dye concentration in phosphate buffer saline, equivalently washed, was used as a control. A. thaliana roots, grown on Murashige and Skoog medium, were incubated with DiD-OMVs or control dye/buffer mixture, washed and observed under confocal laser scanning microscope. Red signals were observed in root hairs and epidermis in DiD-OMV treated plants, while in control-treated roots the same signals were missing.
engleski
2023
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outer membrane vesicles (omvs); Paraburkholderia phytofirmans Ps-JN; intercellular communication