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Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts
Čutović, Natalija
Batinić, Petar
Markovic, Tatjana
Radanovic, Dragoja
Bugarski, Branko
Marinkovic, Aleksandar
Jovanović, Aleksandra A.
Winter savory (Satureja montana L.) is a widely known medicinal plant species, which has been cultivated from seeds at the experimental field of the Institute “Dr Josif Pančić”, Pančevo, Serbia. The plant material used in this study was harvested from a 6-year-old plant. The aim of the study was to optimize the standard maceration process for polyphenol extraction from air-dried plant material, utilizing 50% ethyl alcohol solution. The optimization was performed through varying two most important parameters for maceration: solid-to-solvent ratio and extraction time. The obtained extracts were characterized via analyzing the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential (ABTS, DPPH, FRAP, and CUPRAC assays), extraction yield, conductivity, density, surface tension, and viscosity. The TPC varied from 7.15±0.8 to 92.2±2.5 mg gallic acid equivalent (GAE)/g of plant material. Further, the extraction time and solid-to-solvent ratio had significant influence on TFC; the highest values were achieved between 30 and 90 min, and at a 1:40 ratio. The highest ABTS●+ and DPPH● radical scavenging activities were detected in the extracts prepared using 1:30-1:50 ratios, while the best ferric and cupric ion reducing antioxidant potential was measured in the extract obtained at a 1:50 ratio. The highest extraction yield was detected in the extract obtained at a 1:50 ratio (86.0±2.9%), whereas the extraction time did not have a statistically significant influence on the mentioned parameter. On the other hand, the highest value of conductivity was measured in the extract prepared at a 1:10 ratio, and the highest density and surface tension of the extract was obtained at a ratio of 1:40 (0.941±0.002 g/mL and 29.0±0.1 mN/m, respectively), while the highest viscosity was measured in the extract prepared at a 1:20 ratio (2.89±0.02 mPa·s). In the present study, the optimization of the extraction was performed, in specific, the extraction time and solid-to-solvent ratio levels for improving the efficiency of polyphenols extraction from S. montana were determined. Our study shows the possibilities for production of polyphenols-rich extract of S. montana which might be used in pharmaceutical, food or cosmetic products.
engleski
2022-10
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