Naslov (eng)

Extracellular vesicles derived from mesenchymal stem/stromal cells derived from dental pulp of exfoliated teeth induce osteogenic differentiation

Autor

Milivojević, Marija
Kosanović, Maja
Bekić, Marina
Čolić, Miodrag
Janaćković, Djordje
Tomić, Sergej

Publisher

Opis (eng)

ABSTRACT INTRODUCTION: Large bone defects in the field of orthopedic surgery remains challenging, primarily due to restricted regenerative capacity of bone tissue. The biomimetic bone implants could improve the osteoregeneration process, but the optimal composition for biomimetics remains unknown. Considering the great potential of extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) in regenerative medicine, our aim was to investigate the osteoinductive potential of EVs derived from MSCs from the dental pulp of human exfoliated deciduous teeth (SHEDs) either undifferentiated or those undergone differentiation into osteoblasts (osteoSHED-EVs), for potential use in biomimetic bone implants. EXPERIMENTAL: SHEDs were isolated from the dental pulp of 5 different donors of deciduous teeth and subjected to characterization by Flow cytometry (FCM). EVs-SHED and EVs-osteoSHED were isolated by sequential ultracentrifugation, and analysed by Western blot, NTA, and electron microscopy. Uptake of PKH67-labelled EVs by SHED was analysed by confocal microscopy and FCM.

Jezik

engleski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Large bone defects; bone regeneration; extracellular vesicles; internalization

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP