Naslov (eng)

Biodegradable Electrospun PLGA Nanofibers-Encapsulated Trichinella Spiralis Antigens Protect from Relapsing Experimental Autoimmune Encephalomyelitis and Related Gut Microbiota Dysbiosis

Autor

Sabljić, Ljiljana
Radulović, Nataša
Djokić, Jelena
Stojanović, Dušica B.
Radojević, Dušan
Glamočlija, Sofija
Dinić, Miroslav
Golić, Nataša
Vasilev, Saša
Uskoković, Petar
Sofronić-Milosavljević, Ljiljana
Gruden-Movsesijan, Alisa
Tomić, Sergej

Publisher

Opis (eng)

ABSTRACT Purpose: Trichinella spiralis has evolved complex immunomodulatory mechanisms mediated by excretory-secretory products (ESL1) that enable its survival in the host. Consequently, ESL1 antigens display excellent potential for treating autoimmune diseases such as multiple sclerosis (MS). However, whether timely controlled delivery of ESL1 antigens in vivo, as in natural infections, could enhance its therapeutic potential for MS is still unknown. Methods: To test this, we encapsulated ESL1 antigens into biodegradable poly (lactide-co-glycolic) acid (PLGA) nanofibers by emulsion electrospinning as a delivery system and assessed their release dynamics in vitro, and in an animal MS model, experimental autoimmune encephalomyelitis (EAE), induced 7 days after PLGA/ESL1 subcutaneous implantation. PLGA/ESL1 effects on EAE symptoms were monitored along with multiple immune cell subsets in target organs at the peak and recovery of EAE. Gut barrier function and microbiota composition were analyzed using qPCR, 16S rRNA sequencing, and metabolomic analyses.

Jezik

engleski

Datum

2025

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC 4.0 - Creative Commons Autorstvo - Nekomercijalno 4.0 International License.

http://creativecommons.org/licenses/by-nc/4.0/legalcode

Predmet

electrospinning, PLGA nanofibers, drug delivery, tolerogenic cells, immune modulation, gut microbiota

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP