Naslov (eng)

Trichinella spiralis extracellular vesicles induce anti-inflammatory and regulatory immune responses in vitro

Autor

Glamočlija, Sofija
Kosanović, Maja
Gruden-Movsesijan, Alisa
Radulović, Nataša
Đokić, Jelena
Tomić, Sergej
Sabljić, Ljiljana

Publisher

Opis (eng)

ABSTRACT The helminth Trichinella spiralis, through its excretory-secretory (ES L1) products, induces immune regulatory mechanisms that modulate the host's immune response not only to itself, but also to bystander antigens, foreign or self in origin, which can result in the alleviation of inflammatory diseases. Under the influence of ES L1, dendritic cells (DCs) acquire a tolerogenic phenotype and the capacity to induce Th2 and regulatory responses. Since ES L1 products represent a complex mixture of proteins and extracellular vesicles (TsEVs) the aim of this study was to investigate the impact of TsEVs, isolated from ES L1 products, on phenotypic and functional characteristics of DCs and to elucidate whether TsEVs could reproduce the immunomodulatory effects of the complete ES L1 product. Monocyte-derived DCs treated with TsEVs acquired semi-matured phenotypes, characterized by low expression of human leukocyte antigen - DR isotype (HLA-DR), cluster of differentiation (CD) 86 (CD86), and CD40, moderate expression of CD83 and C-C chemokine receptor type 7 (CCR7), and increased expression of tolerogenic markers indoleamine 2,3-dioxygenase 1 (IDO-1) and immunoglobulin-like transcript 3 (ILT3), together with the unchanged production of IL-12 and IL-23, and elevated production of IL-10 and transforming growth factor (TGF)-β, compared with controls.

Jezik

engleski

Datum

2025

Licenca

© All rights reserved

Predmet

ES L1 products; Exosomes; Extracellular vesicles; Immunomodulation; Regulatory T cells; Tolerogenic dendritic cells; Trichinella spiralis

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP