Naslov (eng)

Isolation of extracellular vesicles from resistant tumor cells using nanobodies-based immunoaffinity approach

Autor

Filipović, Lidija
Tursunović, Marija
Kosanović, Maja
Grozdanić, Marija
Podolski-Renić, Ana
Pešić, Milica
Popović, Milica

Publisher

Opis (eng)

ABSTRACT Introduction: Extracellular vesicles (EVs) are an important contributing factor to drug resistance in cancer. In order to study their features and elucidate their molecular composition. To that extent, we have decided to use two pairs of multi-drug resistant (MDR) cancer cell lines (non-small cell lung carcinoma NCI-H460/R and glioblastoma U87-TxR) and their sensi- tive counterparts (H460 and U87, respectively ion and contribution in drug resistance, EVs need to isolated in an efficient manner and sufficient quantitives. Broadening new possibili- ties in EV-based diagnostics requires innovative, adaptable, and affordable methods for the scalable isolation of high-purity EVs from different sources. This study aims to adapt high-performance immune capture chromatography based on nanobody technology for EVs isolation from cell culture media of MDR cancer cells and their sensitive cells.

Jezik

engleski

Datum

2024

Licenca

© All rights reserved

Predmet

MDR; extracellular vesicles; nanobody technology

Deo kolekcije (1)

o:25140

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