Naslov (eng)

Probing the effects of dextran-coated CeO2 nanoparticles on lung fibroblasts using multivariate single-cell Raman spectroscopy

Autor

Mićević, Mirjana
Čalija, Sonja
Korićanac, Lela
Žakula, Jelena
Vilotić, Aleksandra
Radović, Marko
Golić, Igor
Korać, Aleksandra
Nacka-Aleksić, Mirjana
Stojadinović, Bojan
Dohčević-Mitrović, Zorana

Publisher

Opis (eng)

ABSTRACT In this study, we investigated the cytotoxic effect of highly soluble dextran-coated CeO2 nanoparticles on human fetal lung fibroblasts MRC-5. We examined individual nanoparticle-treated cells by Raman spectroscopy and analyzed Raman spectra using non-negative principal component analysis and k-means clustering. In this way, we determined dose-dependent differences between treated cells, which were reflected through the intensity change of lipid, phospholipid and RNA-related Raman modes. Performing standard biological tests for cell growth, viability and induction of apoptosis in parallel, these changes were correlated with nanoparticle-induced apoptotic processes. The cells with specific spectral characteristics, referring to non-apoptotic, but possibly autophagic cell death modality, were also detected. Additionally, Raman imaging combined with principal component and vertex component analysis was used to map the spatial distribution of biological molecules in treated and untreated cells. This work provided the description of different resulting states of the treated cells depending on the dextran-coated CeO2 nanoparticles dose, which can be later used in the design of the nanoparticles for industrial or medical applications. The wide content of information resulting from single-cell Raman spectroscopy has the potential to detect biochemical changes caused by nanoparticles that would otherwise require a series of expensive and time-consuming standard biological techniques.

Jezik

engleski

Datum

2025

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-ND 4.0 - Creative Commons Autorstvo - Nekomercijalno - Bez prerada 4.0 International License.

http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode

Predmet

CeO2; MRc-5 fibroblasts; Raman spectroscopy; PCA; VCA

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP