Naslov (eng)

Advancing Immunoassay Precision: A Novel Preanalytical Method for Enhancing Thyroglobulin Measurement in the Presence of Tg Antibodies

Autor

Djorić, Ilona
Todorović, Aleksandra
Gnjatović, Marija
Golubović, Snežana
Žarković, Miloš
Janković Miljuš, Jelena
Išić Denčić, Tijana
Šelemetjev, Sonja

Publisher

Opis (eng)

ABSTRACT Thyroglobulin (Tg) is a reliable marker for detecting recurrence in differentiated thyroid cancer (DTC) patients, but frequently occurring Tg antibodies (TgAbs) can hinder accurate measurement. We aimed to develop a preanalytical protocol for precise Tg detection in TgAb presence using the immunoradiometric assay (IRMA) platform. This study involved forty-five patients who underwent IRMA Tg and radioimmunoassay (RIA) TgAb measurements, including two patients monitored for recurrence and one with confirmed recurrence. All three had undetectable Tg levels. We evaluated three preanalytical methods in aiming to separate Tg from TgAbs: buffer only (Protocol 1), micro-spin filters only (Protocol 2), and a combination of both (Protocol 3).

Jezik

engleski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Tg; Tg immunoassays; IRMA; preanalytical treatment; TgAb interference; DTC follow-up; recurrent DTC

Deo kolekcije (1)

o:25140

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