Naslov (eng)

Optimizing parameters related to the phenolic and flavonoid contents of Steepe peony leaves

Autor

Batinić, Petar
Cutovic, Natalija
Jovanović, Aleksandra A.
Marković, Tatjana

Opis (eng)

The current study had two objectives: (1) the determination of the total phenolic content (TPC) and total flavonoid content (TFC) of steppe peony leaf extracts prepared in two different extraction methods, maceration using conventional orbital shaker (MCOS) and ultrasound-assisted extraction (UAE) and (2) optimization of the extraction process parameters via varying solvent type (aqueous, and ethanolic (50% and 70%, v/v), time of extraction (5, 15, 30, and 60 min), and solid-to-solvent ratio (1:10, 1:20, 1:30, 1:40, and 1:50). The total phenolics were extracted most effectively by UAE (411,3 mg gallic acid equivalents (GAE)/mL of raw extract). The MCOS gave lower values, approximately 112,9 mg GAE/mL of raw extract. Total flavonoids content were ranged from 3,0 to 20,0 mg catechin equivalents (CE)/mL of raw extract for UAE, and from 2,98 to 22,87 mg CE/mL of raw extract for MCOS. For all tested analytes, it was observed that a concentration of ethanol of 50% (v/v), a time of extraction of 15 min, and the solid-to-solvent ratio of 1:20 were the most effective for extracting bioactive substances (phenolics and flavonoids) from Steppe peony.

Jezik

engleski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-SA 4.0 - Creative Commons Autorstvo - Nekomercijalno - Deliti pod istim uslovima 4.0 International License.

http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode

Predmet

phenolics, colorimetric determination, gallic acid equivalents, conventional and „green“ extraction

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP