Naslov (eng)

Ultrasound and natural deep eutectic solvents as tools for improving extraction yield and antioxidant potential of wild thyme extracts

Autor

Plećić, Ana
Rakin, Sandra
Jovanović, Aleksandra A.
Gnjatović, Marija
Mladjenović, Lidija

Opis (eng)

The aerial part of wild thyme has widespread use in traditional medicine for treating gastrointestinal and respiratory disorders, rheumatism, menstrual pain, wounds, and eczema. Its extracts possess antioxidative, anti-inflammatory, antihypertensive, antibacterial, and antifungal properties. Ultrasound extraction is a novel procedure used to isolate various plant bioactive compounds, due to the increased extraction yield, fast kinetics, and simple operation. Furthermore, natural deep eutectic solvents can increase phenolic recovery from plant matrix. In the present research, the extracts were prepared using dried wild thyme herb and water, 35% ethanol, or two types of natural deep eutectic solvents with 50 % water - betaine+citric acid and citric acid+saccharose in an ultrasound bath. The obtained extracts were characterized via analyzing the total phenolic and flavonoid contents (TPC and TFC, respectively) and antioxidant capacity (ABTS⦁+ and DPPH⦁ assays). The TPC of water, ethanol, betaine+citric acid, and citric acid+saccharose extracts were 25.08±2.3, 27.5±1.2, 30.2±2.9, and 28.1±3.3 mg gallic acid equivalents (GAE)/g of dried plant material, respectively. At the same time, the TFC values amounted to 10.5±0.8, 13.1±1.3, 1.0±0.5, and 2.8±0.1 mg catechin equivalents (CE)/g of dried plant material, respectively. In the ABTS⦁+ assay, antioxidant activity was 7.7±1.0 µmol Trolox equivalent (TE)/g of dried plant material (water extract), 8.5±0.9 µmol TE/g (ethanol extract), 10.8±0.11 µmol TE/g (betaine+citric acid extract), and 17.0±0.7 µmol TE/g (citric acid+saccharose extract). DPPH radical scavenging capacity was expressed as IC50 (concentration of the extract required to neutralize 50% of free DPPH radicals); IC50 was 4.3±0.2 mg/mL for water extract, 4.0±0.5 mg/mL for ethanol extract, 3.5±0.2 mg/mL for betaine+citric acid extract, and 2.9±0.1 mg/mL for citric acid+saccharose extract. pH values for ethanol, betaine+citric acid, and citric acid+saccharose extracts were 6.97, 6.18, 2.47, and 1.60, respectively. Due to higher ABTS⦁+ and DPPH⦁ scavenging activity, wild thyme extract obtained using citric acid and saccharose with 50% water compared to water, ethanol, and betaine+citric acid extracts was favored as an ingredient in food and pharmaceutical products.

Jezik

engleski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-SA 4.0 - Creative Commons Autorstvo - Nekomercijalno - Deliti pod istim uslovima 4.0 International License.

http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode

Predmet

antioxidants, natural deep eutectic solvents, phenolics, ultrasound, wild thyme

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP