Naslov (eng)

Antioxidant capacity of silibinin-loaded liposomes

Autor

Karkad, Amjed
Batinić, Petar
Milošević, Milena
Pirković, Andrea
Onjia, Antonije
Marinković, Aleksandar
Jovanović, Aleksandra A.

Opis (eng)

Silibinin is the major active constituent of silymarin, a standardized extract of milk thistle seeds. Silibinin has been used traditionally as a chemopreventive and therapeutic agent in human lung cancer. It also possesses hepatoprotective, antioxidant, hypocholesterolemic, antitumor, cardioprotective, neuroprotective, and antiviral activities. However, its application is limited due to poor water solubility, intestinal resorption, and low bioavailability. Liposomes, as non-toxic, biodegradable, and biocompatible lipid carriers, can provide controlled delivery of bioactive components and their protection from degradation caused by light, oxygen, UV irradiation, different pH values, and enzymes. Additionally, phospholipids from the liposomes do not provoke a reaction with taste receptors, and, therefore, the liposomal bilayer is an appropriate carrier for covering the unpleasant taste of numerous polyphenols. With the aim of investigating the antioxidant potential of silibinin-loaded liposomes after different technological procedures (UV irradiation and lyophilization), two antioxidant assays were employed (ABTS and DPPH tests). Liposomes with silibinin were prepared using the proliposome method and phospholipids. According to the results of the ABTS test, the antioxidant activity of pure silibinin was 0.769 µmol Trolox equivalent (TE)/mL, while the antioxidant potential was lower after the encapsulation in liposomes; 0.548 µmol TE/mL after preparation, 0.549 µmol TE/mL after UV irradiation, and 0.436 µmol TE/mL after lyophilization. Furthermore, the DPPH radical scavenging activity was 20.97% for pure silibinin, 22.48% immediately after the liposomal preparation, 22.24% after UV irradiation, and 18.73% after lyophilization. As can be seen, UV irradiation did not cause significant changes in the overall antioxidant potential of silibinin-loaded liposomes. Nevertheless, lyophilization significantly decreased the radical scavenging activity of the liposomes. Considering that the two used antioxidant assays are based on different principles and reactions, the obtained results provide good insight into the overall antioxidant activity of silibinin-loaded liposomes.

Jezik

engleski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-SA 4.0 - Creative Commons Autorstvo - Nekomercijalno - Deliti pod istim uslovima 4.0 International License.

http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode

Predmet

antioxidant capacity, encapsulation, liposomes, proliposome method, silibinin

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP