Digitalni repozitorijum
Univerziteta u Beogradu
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Assessment of sun protecting factor and in vitro cytotoxicity of methanolic pulp extracts from Serbian Cucurbita maxima in human keratinocytes
Dekanski, Dragana
Jovanović, Aleksandra
Miljić, Milorad
Krstić, Sanja
Pirković, Andrea
Ultraviolet radiation from sun exposure causes harmful skin effects, such as dermal photoaging and DNA damage, mediated by oxidative stress. Pumpkin (Cucurbita maxima) is abundant in carotenoids, polyphenols, and tocopherols which have antioxidant ability and protect cells from damage making them attractive as potential natural photo-protectants for skin applications. Pumpkin pulp, considered as by-product of the food industry, is the least explored in terms of its biological activities, although it contains considerable amounts of bioactive compounds. The aim of this study was to evaluate in vitro sun protecting factor (SPF) and cytotoxicity of methanolic pumpkin pulp extracts (MPE). They were prepared from the material of 4 accessions MAX 113, MAX 118-1, MAX 117, and MAX 1 from the breeding collection of the Institute of Field and Vegetable Crops, Novi Sad, Serbia. These accessions were selected according to the highest carotenoid content and antioxidant capacity. The effect of MPE on human keratinocytes (HaCaT) viability was determined by crystal violet assay. In vitro SPF based on the Mansur equation and the absorbance measurements was chosen to screen the photoprotective potential of MPE. The results show that SPF values for extracts MAX 113, MAX 118-1, MAX 117, and MAX 1 were 2.351, 1.875, 4.573, and 3.812, respectively. The most pronounced in vitro SPF values were in extracts MAX 117 and MAX 1 which were previously shown to contain the high amounts of carotenoids zeaxanthin and β-carotene. In vitro study of cell viability in keratinocytes showed that MAX 118- 1, MAX 117, and MAX 1 did not reduce the number of viable cells up to the concentration of 1000 µg/mL and thus might be considered as non-toxic. Among the analyzed extracts, extract MAX 113 reduced HaCaT cells viability after 24 h incubation in a concentration-dependent manner, where the highest concentration of 1000 µg/mL significantly reduced number of viable cells compared to the non-treated control. On the other hand, the treatment of keratinocytes using extract MAX 117 led to a significant increase in the number of viable cells at 1000 µg/mL concentration. That was the same extract that exhibited the highest SPF value. These data demonstrate that bioactive compounds from MPE could have potential as anti-photoaging agents and can be considered as non-toxic for the skin cells. Additionally, findings suggest MPE of C. maxima Duchesne cultivar MAX 117 from Serbian accession has the best photoprotective potential and could be useful as component in natural cosmetic products.
engleski
2024
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