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Univerziteta u Beogradu
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Ultrasound-assisted extraction of Rosa canina L. using natural deep eutectic solvents
Jovanović, Aleksandra A.
Pjanović, Rada
Živković, Jelena
Šavikin, Katarina
Gnjatović, Marija
Marinković, Aleksandar
Bugarski, Branko
ABSTRACT Rosa canina L. (Rosaceae) is frequently employed in traditional medicine, due to its diuretic, anti-inflammatory, anti-allergic, antioxidant, and analgesic properties. The ultrasound-assisted extraction is frequently used as an extraction technique, due to the increased extraction yield, improved extract quality, fast kinetics, and simple operation. Additionally, in recent times, natural deep eutectic solvents (NADESs) are applied as a tool for improving polyphenol recovery from various plant materials. Thus, in the present study, R. canina extracts were prepared using dried rose hips (0.25 g), two types of natural deep eutectic solvents (25 mL, choline chloride+citric acid with 50% of water and betaine+citric acid with 70% of water), and ultrasound probe (40% amplitude for 10 min). The extracts were characterized via analyzing total polyphenol content (TPC), antioxidant potential (ABTS and DPPH assays), extraction yield, pH, zeta potential, conductivity, density, surface tension, and viscosity. TPC of choline chloride+citric acid and betaine+citric acid extracts were 8.75±0.17 and 7.47±0.04 mg gallic acid equivalents (GAE)/g of plant material, respectively. ABTS radical scavenging activity was 3.03±0.62 mmol Trolox/g of plant material (choline chloride+citric acid extract) and 2.82±0.13 mmol Trolox/g (betaine+citric acid extract), whereas, in DPPH assay, IC50 was 1.02±0.04 mg/mL for choline chloride+citric acid extract and 1.98±0.10 mg/mL for betaine+citric acid extract. The extraction yield was 0.575±0.035% (choline chloride+citric acid extract), and 0.490±0.016% (betaine+citric acid extract). pH values for chloride+citric acid and betaine+citric acid extracts were 0.83±0.03 and 2.68±0.02, while zeta potential was 2.46±0.16 and 0.88±0.09 mV, respectively. Conductivity for choline chloride+citric acid and betaine+citric acid extracts were 35.8±0.4 and 4.3±0.3 mS/cm, density was 1.16±0.01 and 1.54±0.02 g/mL, while surface tension was 35.2±0.8 and 37.2±0.1 mN/m, respectively. Viscosity was 6.52±0.01 mPa•s (choline chloride+citric acid extract) and 5.14±0.12 mPa•s (betaine+citric acid extract). Due to higher TPC and DPPH radical scavenging capacity, R. canina extract prepared using choline chloride and citric acid with 50% water in comparison to betaine+citric acid extract was favored as an ingredient in food, pharmaceutical, and cosmetic products.
engleski
2023
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antioxidant activity, natural deep eutectic solvent, polyphenols, Rosa canina, viscosity