Naslov (eng)

Extremely low-frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5

Autor

Leković, Maida
Dreković, Nerkesa
Granica, Nihat
Mahmutović, Elvis
Djordjević, Natasa

Opis (eng)

Abstract: Numerous studies have shown that extremely low-frequency electromagnetic field (ELF-EMF) by modulating oxidativeantioxidative balance in the cells achieved beneficial and harmful effects on living organisms. The aim of this study was to research changes of both proliferative capacity and redox homeostasis of human lung fibroblast cell lineMRC-5 during exposure to ELF-EMF (50 Hz). The human lung fibroblast cell lineMRC-5 were exposed to ELF-EMF once a day in duration of 1 h during 24 h (1 treatment 1 h/day), 48 h (2 treatments 1 h/day), 72 h (3 treatments 1 h/day), and 7 days (7 treatments 1 h/day). After 24 h of the last treatment, the proliferative capacity of the cells and the concentrations and activities of the components of the oxidative/ antioxidative system were determined: superoxide anion (O2 .−), hydrogen peroxide (H2O2), nitric oxide (NO), peroxynitrite (ONOO−), reduced glutathione (GSH), oxidized glutathione (GSSG), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione-S-transferase (GST). The results of this study show that ELFEMF may affect a cell cycle regulation of human lung fibroblast cell lineMRC-5 through modulation of oxidative/antioxidative defense system. The effects of ELF-EMF on proliferation and redox balance of human lung fibroblast cell lineMRC-5 depend on exposure time.

Jezik

engleski

Datum

2020

Licenca

© All rights reserved

Predmet

Keywords: Electromagnetic field, Redox homeostasis, Cell proliferation, Human lung fibroblasts

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