Naslov (eng)

The association of CAT-262C/T polymorphism with catalase activity and treatment response in juvenile idiopathic arthritis

Autor

Pavlović, Dušica
Vojinović, Jelena
Bašić, Jelena
Despotović, Milena
Jevtović-Stoimenov, Tatjana
Cvetković, Tatjana
Cvetković, Mina
Milošević, Vuk
Sušić, Gordana

Opis (eng)

Abstract: Oxidative stress is believed to be of great importance for both the etiology and the persistence of juvenile idiopathic arthritis (JIA). The aim of this study was to investigate the association of -262C/T polymorphism of the catalase (CAT) gene with JIA, as well as to evaluate whether this polymorphism can influence plasma CAT activity and outcome in JIA patients treated with etanercept. A total of 154 subjects (60 JIA patients and 94 healthy volunteers) were screened for CAT-262C/T gene polymorphism using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. Plasma CAT activity was determined using the spectrophotometric method according to Goth, prior to and 12 months after anti-TNF (etanercept) therapy. Clinical outcome was assessed using the JIA ACR (American College of Rheumatology) response criteria. The genotype and allele frequency distributions of CAT-262C/T polymorphism in the patients were signifcantly different from those of the controls (p = 0.014, p = 0.006). The TT genotype (polymorphic homozygous) was associated with a 4.36-fold higher likelihood of Državni univerzitet u Novom Pazaru having JIA (95% CI 1.545–12.323, p = 0.005) as compared to the CC genotype (wildtype). At month 12 of treatment, JIA patients, carriers of the CC genotype, showed signifcantly higher plasma CAT activity (p = 0.004) and achieved the JIA ACR 70 response more often (p= 0.003) than the patients, carriers of the CT/TT genotype. This is the frst study implying the possible association of CAT-262C/T polymorphism with JIA. The results suggest the potential protective effect of the CC genotype, with regard to CAT activity and treatment outcome.

Jezik

engleski

Datum

2019

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Keywords: Catalase · CAT-262C/T polymorphism · Etanercept · Juvenile idiopathic arthritis

Deo kolekcije (1)

o:28516

Radovi nastavnika i saradnika Državnog univerziteta u Novom Pazaru