Naslov (eng)

HPLC, UV-Vis and NMR spectroscopic and DFT characterization of purpurin isolated from Rubia tinctorum L.

Autor

Marković, Zoran
Manojlović, Nedeljko
Jeremić, Svetlana
Živić, Miroslav

Opis (eng)

Abstract: Purpurin (1,2,4-trihidroxyanthraquinone), a naturally occuring anthraquinone pigment present in the root of Rubia tinctorum (Mull. Arg.) Zahlbr. was isolated from the plant, purified and characterized by HPLC chromatography, UV-vis and NMR spectroscopy. The geometries of the purpurin conformers were optimized using the B3LYP/6-311+G(d,p) level of theory. It was found that all conformations have similar energies and that the corresponding purpurin radicals are planar. In spite of their planarity, there is no significant electron delocalization over the A and C rings. The antioxidant properties of purpurin were investigated using the colorimetric assay as Trolox-equivalent antioxidant capacity, and theoretical BDE (Bond Dissociation Enthalpy). Both methods revealed that purpurin has strong antioxidant capacity.

Jezik

engleski

Datum

2018

Licenca

© All rights reserved

Predmet

Keywords: Rubia tinctorum; HPLC-UV; antioxidant activity; anthraquinone; DFT study

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o:28516

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