Naslov (eng)

MiR-375 and miR-21 as potential biomarkers of prostate cancer: comparison of matching samples of plasma and exosomes

Autor

Matijašević Joković, Suzana
Brajušković, Goran
Savić-Pavićević, Dušanka
Vuković, Ivan
Korać, Aleksandra
Popović, Milica
Filipović, Lidija
Kotarac, Nevena
Dobrijević, Zorana

Publisher

Opis (eng)

ABSTRACT MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa.

Jezik

engleski

Datum

2022

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

prostate cancer, exosomes, plasma, microRNAs, miR-21, miR-375

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP