Naslov (eng)

Efficacy of anaerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat

Autor

Vasilev, Saša
Vasilev, Dragan
Sofronić Milosavljević, Ljiljana
Karabasil, Nedjeljko
Sabljić, Ljiljana
Mitić, Ivana
Suvajdžić, Branko

Publisher

Opis (eng)

ABSTRACT Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.

Jezik

engleski

Datum

2022

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Trichinella, digestion method, pepsin

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP