Naslov (srp)

Uticaj postpartalne aplikacije kalcijuma na metaboličku adaptaciju hipokalcemičnih krava : doktorska disertacija

Autor

Palamarević, Milija, 1988-

Doprinosi

Sladojević, Željko, 1969-
Kirovski, Danijela, 1969-
Vujanac, Ivan, 1971-

Opis (eng)

to the clinically visible form, there is a subclinical form that is difficult to detect due to theabsence of a clinical signs. Timely treatment of cows is crucial for the sustainability of production andhealth of cows after calving. Data from the literature are inconsistent regarding the outcome of therapy,which is why we considered important to examine the mechanisms underlying the therapeuticprocesses applied in hypocalcemic cows. The aim of this study was to determine the prevalence ofsubclinical hypocalcemia and to examine the effect of oral and parenteral administration of Capreparation on the activity of the parathyroid gland, metabolic parameters in blood of postpartum cowswith subclinical hypocalcemia. The study included 180 cows from the 3rd to the 7th lactation, fromwhich blood was taken 5 to 15 hours after calving in order to determine the level of blood calcium.Cows (n = 24) with subclinical hypocalcemia (1.38 mmol/l ≥Ca ≤ 2.0 mmol/l) were divided into threegroups: (1) IV-Ca (n = 8) treated once by intravenous administration of 500 ml Calci-kel 300 (12.5 gCa gluconate, 2.4 g Mg chloride and boric acid), (2) PO-Ca (n = 8) treated twice (1l when diagnosingthe disease and 0.5 l 12 hours later) oral suspension of Calci Tonic (chelation bounded Ca and Mg ions,respectively 41g Ca and 5g Mg per liter), (3) KON (n = 8) was not treated with Ca and Mg.Blood was sampled at hour 0 (moment of diagnosis of subclinical hypocalcemia, immediatelybefore treatment) as well as at 1, 2, 4, 8, 12, 20, 24, 36 and 48 hours. Urine samples were taken at 0, 1,12, 24, 36, and 48 hours. Calcemia was determined in all blood samples. The concentration ofparathyroid hormone (PTH) in the blood was measured at 0., 1., 2., 4., 24. and 48 hours.Concentrations of glucose, total proteins, albumin, urea, creatinine, total bilirubin, BHBA, inorganicphosphorus, magnesium and enzyme activity of gGT, AST and ALP were determined in blood samplestaken at 0, 24, and 48 hours, and the concentration of NEFA only at hour 0. Urine pH was determinedin all urine samples.The overall prevalence of subclinical hypocalcemia was 13%, with the highest in the fourth(42%) and fifth (37%) and lowest in the third lactation (3.03%).Before treatment, there was no significant difference in calcemia between the groups, while inrelation to the KON group, calcemia was significantly higher after treatment in PO-Ca (up to 8 hours)and IV-Ca (up to 24 hours). In the PO-Ca group, physiological calcemia was established during thefirst 2 hours after treatment and was maintained until the end of the study, while in the IV-Ca group,hypercalcemia occurred in the first two hours after treatment, and then normocalcemia was establisheduntil the end of the study. Calcemia of the KON group was below the lower physiological limit untilthe hour 48th from the beginning of the experiment.Before treatment, there was no significant difference in PTH concentrations between groups,while after treatment the values were significantly lower compared to the KON group in IV - Ca duringfirst two hours, and in both treated groups (PO-Ca, IV-Ca) in the fourth hour and then significantlylower PTH was established in PO-Ca, compared to KON, until the end of the study.Based on the measured initial values of urine pH, metabolic alkalosis was established in allcows. After one hour, acidosis was found in group IV-Ca, with the urine pH in this and PO-Ca groupsbeing significantly lower than in the KON group. Twelve hours after treatment, urine pH of PO-Ca andIV-Ca groups were significantly lower than in KON group. In the PO-Ca group, the pH of urine wassignificantly lower than in the KON group at 24 and 36 hours, while at hour 48 there was no differencebetween the pH of urine of the examined groups.Concentrations of glucose, BHBA, NEFA and creatinine, as well as GGT and ALP enzymeactivities did not differ significantly between the examined groups of cows during the study...

Opis (srp)

Hipokalcemija je jedan od najučestalijih metaboličkih postpartalnih poremećaja kod krava.Pored klinički vidljive forme, javlja se supklinički oblik koji je teško detektovati zbog izostankakliničke slike. Pravovremeni tretman ovih jedinki je ključan za održivost proizvodnje i zdravlja kravaposle teljenja. Podaci iz literature su neusaglašeni u pogledu ishoda terapije, zbog čega smo smatrali daje značajno ispitati mehanizme koji se nalaze u osnovi terapijskih procesa koji se primenjuju kodhipokalcemičnih krava. Cilj ovog rada je bio da se utvrdi prevalenca supkliničke hipokalcemije i ispitaefekat peroralne i parenteralne aplikacije preparata Ca na aktivnost paratireoidne žlezde, metaboličkeparametre u postpartalnih krvi krava sa supkliničkom hipokalcemijom. U ispitivanje je uključeno 180krava od 3. do 7. laktacije, kojima je 5 do 15 sati posle teljenja određena kalcemija. U ogled su uvedenekrave (n= 24) sa supkliničkom hipokalcemijom (1,38 mmol/l ≥Ca ≤ 2,0 mmol/l), podeljene u tri grupe:(1) IV-Ca (n=8) jednokratno tretirana intravenskom aplikacijom 500 ml Calci–kel 300 (12,5 g Caglukonata, 2,4 g Mg hlorida i borna kiselina), (2) PO-Ca (n=8) dvokratno tretirana (1 l pridijagnostikovanju oboljenja i 0,5 l 12 sati kasnije) oralnom suspenzijom Calci Tonica (helatno vezanijoni Ca i Mg, odnosno 41g Ca i 5g Mg po litru), (3) KON (n=8) nije tretirana preparatima Ca i Mg.Krv je uzorkovana 0. časa (momenat dijagnostikovanja supkliničke hipokalcemije, neposrednopre tretmana) kao i 1., 2., 4., 8., 12., 20., 24., 36. i 48. sata. Uzorci urina su uzimani 0., 1., 12., 24., 36. i48. sata. Kalcemija je određivana u svim uzorcima krvi. Koncentracija parathormona (PTH) u krvi jemerena 0., 1., 2., 4., 24 . i 48. sata. Koncentracija glukoze, ukupnih proteina, albumina, uree, kreatinina,ukupnog bilirubina, BHBA, anorganskog fosfora, magnezijuma i aktivnost enzima gGT, AST i ALPodređivana je u uzorcima krvi uzetim 0., 24., i 48. sata, a koncentracija NEFA samo 0. sata. pH urina jeodređen u svim uzorcima urina.Ukupna prevalenca supkliničke hipokalcemije je bila 13%, pri čemu je bila najveća u četvrtoj(42%) i petoj (37%), a najniža u trećoj laktaciji (3,03%).Pre tretmana nije bilo značajne razlike u kalcemiji između grupa, dok je u odnosu na KONgrupu kalcemija bila značajno veća nakon tretmana kod PO-Ca (do 8. časa) i IV-Ca (do 24. časa). KodPO-Ca grupe fiziološka kalcemija uspostavljena je tokom prva 2 sata nakon tretmana i održavala se dokraja ogleda, dok je kod IV-Ca grupe prva dva sata nakon tretmana došlo do hiperkalcemije, a zatim jeuspostavljena normokalcemija do kraja ogleda. Kalcemija KON grupe bila je ispod donje fiziološkegranice do 48. sata od početka ogleda.Pre tretmana nije bilo značajne razlike u koncentraciji PTH, dok su nakom tretmana vrednostibile značajno niže u odnosu na KON grupu kod IV-Ca prvog i drugog sata a kod obe tretirane grupe(PO-Ca, IV-Ca) četvrtog sata i zatim kod PO-Ca sve do kraja ogleda.Na osnovu izmerenih početnih vrednosti pH urina, kod svih krava je ustanovljena metaboličkaalkaloza. Nakon jednog sata kod IV-Ca grupe ustanovljena je acidoza, pri čemu je pH urina kod ove iPO-Ca grupe bio značajno niži nego kod KON grupe. Dvanaest sati nakon tretmana, pH urina PO-Ca iIV-Ca grupe bio je značajno niži nego kod KON grupe. Kod PO-Ca grupe pH urina je bila značajnoniža nego kod KON grupe 24. i 36. sata, dok 48. sata između pH urina ispitivanih grupa nije bilorazlike.Koncentracije glukoze, BHBA, NEFA i kreatinina, kao i aktivnosti enzima GGT i ALP nisu seznačajno razlikovale između ispitivanih grupa krava tokom ogleda.Na početku ogleda kod svih krava je ustanovljena hipofosfatemija. Nakon 48 sati, kod IV-Cagrupe vrednost aP je bila značajno niža nego kod KON grupe, dok se kod PO-Ca grupe nije razlikovalaod vrednosti kod KON grupe...

Opis (srp)

Veterinarska medicina - Bolesti papkara / Veterinary medicine - Ruminants and swine disease Datum odbrane: 17.01.2022.

Jezik

srpski

Datum

2021

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-ND 3.0 AT - Creative Commons Autorstvo - Nekomercijalno - Bez prerada 3.0 Austria License.

http://creativecommons.org/licenses/by-nc-nd/3.0/at/legalcode

Predmet

OSNO - Opšta sistematizacija naučnih oblasti, Interna veterinarska medicina

cows, hypocalcemia, parathyroid hormone, therapy

OSNO - Opšta sistematizacija naučnih oblasti, Interna veterinarska medicina

krave, hipokalcemija, parathormon, terapija

619:616-008:636.2(043.3)