Naslov (eng)

IgM and IgG immunoreactivity of SARS-CoV-2 recombinant M protein

Autor

Lopandić, Zorana
Gavrović-Jankulović, Marija
Ćujić, Danica
Gnjatović, Marija
Glamočlija, Sofija
Todorović, Aleksandra
Protić-Rosić, Isidora

Publisher

Opis (eng)

ABSTRACT Diagnostic evaluation of specific antibodies against the SARS CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.

Jezik

engleski

Datum

2021

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

SARS-CoV-2 membrane protein, COVID-19, IgM reactivity, IgG reactivity, antigenicity, ELISA

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP