Naslov (eng)

Lectin-Based Protein Microarray for the Glycan Analysis of Colorectal Cancer Biomarkers: The Insulin-Like Growth Factor System

Autor

Robajac, Dragana
Katrlík, Jaroslav
Nedić, Olgica
Gemeiner, Peter
Miljuš, Goran
Šunderić, Miloš
Križáková, Martina

Publisher

Opis (eng)

ABSTRACT Lectin-based protein microarrays are used for glycoprofiling of various kinds of biological samples. Here we describe lectin-based microarray assay in the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied the described method for glycan analysis of glycoprotein biomarkers of colorectal cancer associated with the insulin-like growth factor system.

Opis (srp)

Sažetak

Jezik

engleski

Datum

2022

Licenca

© All rights reserved

Predmet

biomarker, cancer, glycosylation, insulin-like growth factor system, lectin-based protein microarray

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP