Naslov (eng)

Serbian external quality assessment for Trichinella detection in meat in 2021 compared to 2017

Autor

Mitić, Ivana
Sofronić-Milosavljević, Ljiljana
Vasilev, Saša
Ilić, Nataša

Publisher

Opis (eng)

ABSTRACT In 2021, the Serbian National Reference Laboratory for Trichinellosis, Serbian Institute for the Application of Nuclear Energy, organized external quality assessment (EQA 2021) for the detection of Trichinella larvae presence in meat by the magnetic stirrer method (MSM). The aims of this study were to examine the performance of the accredited laboratories over time and to compare the participants’ performance. EQA 2021 was organized according to ISO 17043, and the test panel consisted of three meat balls, two of which were spiked with four Trichinella spiralis L1 larvae. Evaluation of the qualitative results showed that 90.91% (100% in 2017) of participants successfully passed the EQA. Quantitative evaluation showed that, on average, 71.59% (in 2017 only 60%) of the spiked Trichinella larvae were detected. This study enabled comparisons of laboratories over time (2017 and 2021) and across the country. The results obtained should serve as motivation for improvement of laboratory performance. All official laboratories with accredited MSM for Trichinella detection should participate in an EQA every second year and all other laboratories that perform Trichinella testing should participate annually in EQAs organized at national level. Regular participation will bring improvement in sensitivity of the test method used and will promote the important one health concept.

Opis (srp)

SAŽETAK U 2021 godini Nacionalna referentna laboratorija za trihinelozu (NRLT) je organizovala eksternu proveru kvaliteta (EPK) za otkrivanje larvi trihinela u mesu korišćenjem metode magnetne mešalice za zbirni uzorak (MMM). Cilj je bio da se omogući zainteresovanim akreditovanim laboratorijama da uporede kvalitet rada tokom vremena, ali i uporede svoj rezultat sa drugim učesnicima. EPK je organizovan prema standardu ISO 17043. Test panel poslat u 11 laboratorija se sastojao od po 3 loptice od mesa sa identičnim brojem larvi. U dva uzorka bilo je dodato po četiri živih mišićnih L1 larvi Trichinella spiralis, a jedan uzorak je bio bez larvi. Procena kvalitativnih rezultata je pokazala da je 90,91% (2017 je bilo 100%) učesnika uspešno prošlo testiranje. Prosečan prinos larvi iznosio je 71,59% (dok je 2017 bilo 60%). Ovaj rad je po prvi put omogućio poredjenje rezultata laboratorija tokom vremena, ali i poredjenje izmedju laboratorija. Učešće u EPK treba da posluži za poboljšanje izvodjenja metode magnetne mešalice za zbirni uzorak. Zato bi bilo poželjno da ove, ali i druge laboratorije u Srbiji učestvuju u narednim EPK ili PT šemama organizovanim na nacionalnom nivou. Cilj je da ove aktivnosti dovedu do poboljšanja senzitivnosti metode, pruže korisne informacije kolegama, ali i Upravi za veterinu i da budu od značaja za promociju koncepta „jedno zdravlje“.

Jezik

engleski

Datum

2021

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 4.0 - Creative Commons Autorstvo 4.0 International License.

http://creativecommons.org/licenses/by/4.0/legalcode

Predmet

Trichinella, external quality assessment 2021, meat

Trichinella, eksterna procena kvaliteta, svinjsko meso

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP