Naslov (eng)

Anti-inflammatory effect of amalgam on periapical lesion cells in culture

Autor

Eraković, Mile
Čolić, Miodrag
Vučević, Dragana
Tomić, Sergej
Milanović, Marijana
Bekić, Marina
Duka, Miloš

Opis (eng)

ABSTRACT Background/Aim. Amalgam has been used for years in dentistry, but the controversy on its adverse effects, both on local oral/dental tissues and systemic health, still exists. When used for retrograde filling in apical surgery, amalgam comes in close contact with the periapical tissue, and it is sometimes responsible for the induction of periapical lesion (PL) or its exacerbation. Therefore, the aim of the study was to examine the effect of amalgam on cytotoxicity and pro-duction of pro-inflammatory cytokine by cells isolated from PL. Methods. Conditioned medium from freshly prepared amalgam (ACM) was performed according to the ISO 10993-12 by incubating the alloy in RPMI medium (0.2 g/mL) for 3 days at 37°C. Cells were isolated from 20 hu-man PLs after apicoectomy by collagenase/DNA-ase diges-tion and cultured with different dilutions of ACM. Cytotox-icity was determined by MTT assay (n = 7 cultures) and apoptosis/necrosis assays (n = 8 cultures), whereas cytokine production was measured by a Flow Cytomix Microbeads Assay (n = 8 cultures). Results. Undiluted (100%) and 75% ACM was cytotoxic due to induction of apoptosis of PL cells. Non-cytotoxic concentrations of ACM (50% and 25%) inhibited the production of pro-inflammatory cyto-kines (TNF-α, IL-1β, IL-6, and IL-8), concentration-dependently. Conclusion. For the first time, our results showed an unexpected anti-inflammatory property of amal-gam on PL cells, which could be beneficial for PL healing after apicoectomy.

Opis (srp)

SAŽETAK Uvod/Cilj. Amalgam se godinama koristi u stomatologiji, ali i dalje postoje kontroverze o njegovim neželjenim efektima na lokalno oralno/dentalno tkivo i sistemsko zdravlje. Kada se koristi za retrogradno punjenje u apikalnoj hirurgiji, amal-gam dolazi u blizak kontakt sa periapeksnim tkivom, što je ponekad povezano sa indukcijom periapeksne lezije (PL) ili njenom egzacerbacijom. Zato je cilj ovog rada bio da se ispita efekat amalgama na citotoksičnost i produkciju pro-inflamacijskih citokina od strane ćelija izolovanih iz PL. Metode. Od sveže napravljenog amalgama pripremljen je kondicionirani medijum (ACM) inkubiranjem legure na 37°C u RPMI medijumu u toku 3 dana (0.2 g/mL) kako je pred-loženo standardom ISO 10993-12. Ćelije su izolovane iz 20 humanih PL nakon apikoektomije, digestijom tkiva pomoću kolagenaze/DNA-aze, a zatim su korišćene za kulturu u pri-sustvu različitih razblaženja ACM. Citotoksičnost je ispitivana pomoću MTT testa (n = 7 kultura) i detekcijom apop-toze/nekroze (n = 8), dok je nivo produkovanih citokina me-ren simultano pomoću eseja sa mikrokuglicama uz pomoć protočne citometrije (n = 8). Rezultati. Nerazblažen ACM (100%) i onaj od 75% pokazali su citotoksični efekat, in-dukujući apoptozu PL ćelija. Necitotoksične koncentracije ACM (50% i 25%) inhibirale su produkciju pro-inflamacijskih citokina (TNF-α, IL-1β, IL-6 i IL-8) na dozno-zavisan način. Zaključak. Naši rezultati po prvi put pokazuju neočekivano antiinflamacijsko svojstvo amalgama na PL ćelije, što može biti korisno za zarastanje lezije nakon apikoektomije.

Jezik

engleski

Datum

2021

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-SA 4.0 - Creative Commons Autorstvo - Deliti pod istim uslovima 4.0 International License.

http://creativecommons.org/licenses/by-sa/4.0/legalcode

Predmet

dental amalgam, periapical tissue, cytokines, cytotoxicity, immunologic, inflammation, apicoectomy

Deo kolekcije (1)

o:25140

Radovi Instituta za primenu nuklearne energije - INEP