Naslov (srp)

Uporedna analiza morfoloških promena i distribucije virusnog antigena u organima labudova i kokošaka prirodno inficiranih virusom avijarne influence podtipa H5N8 : doktorska disertacija

Autor

Božić, Biljana, 1987-

Doprinosi

Aleksić-Kovačević, Sanja, 1965-12679783
Polaček, Vladimir, 1973- 13265767
Vučićević, Ivana, 1978-25067367
Vučićević, Miloš, 1985- 31474791
Petrović, Tamaš, 1969- 13259111

Opis (srp)

Infekcije izazvane visoko patogenim virusima avijarne influence ustanovljene su kod ptica, velikog broja domaćih i divljih životinja i ljudi i iz tog razloga predstavljaju veliku opasnost sa aspekta veterinarske medicine i javnog zdravlja. Izrazita genetička varijabilnost i nestabilnost genoma influenca virusa omogućavaju nastanak velikog broja različitih podtipova virusa avijarne influence sa izmenjenim svojstvima u pogledu antigenskih osobina, patogenosti i specifičnosti prema domaćinu. Novi, resortirani podtip visoko patogene avijarne influence H5N8 prvi put je dijagnostikovan na evropskom kontinentu 2014. godine. U Republici Srbiji, prva zabeležena epizootija avijarne influence podtipa H5N8 desila se krajem 2016. godine. Tokom ove epizootije, najveća stopa mortaliteta zabeležena je kod labudova grbca (Cygnus olor), a pored toga virus je dijagnostikovan i kod malog broja domaćih kokošaka gajenih u seoskim domaćinstvima. Istraživanja u ovoj doktorskoj disertaciji imala su za cilj uporedno ispitivanje makroskopskih i mikroskopskih promena, distribucije i tropizma virusa, kao i određivanje imunofenotipa ćelija inflamacije u organima dve različite vrste ptica prirodno inficiranih virusom avijarne influence podtipa H5N8. U ispitivanjima su korišćeni organi 15 labudova grbaca (red Anseriformes) i 15 kokošaka (red Galliformes) uginulih tokom ove epizootije. Pored toga, cilj istraživanja bio je identifikacija i molekularna karakterizacija virusa avijarne influence detektovanog u Srbiji tokom zime 2016. – 2017. godine. Za dokazivanje prisustva virusa influence u ispitanom materijalu poreklom od prirodno inficiranih ptica korišćena je real time RT-PCR metoda. Za utvrđivanje karaktera lezija, distribucije virusnog antigena i imunofenotipa ćelija inflamacije u tkivu pluća, srca, slezine, creva, bubrega, pankreasa i mozga, primenjena su patohistološka i imunohistohemijska ispitivanja...

Opis (srp)

Veterinarska medicina - Patološka morfologija / Veterinary Medicine- Pathological Morphology Datum odbrane: 25.03.2019.

Opis (eng)

Highly pathogenic avian influenza viruses cause infections in birds, a large number of domestic and wild animals as well as in humans, and are the cause of serious veterinary and public health concern. The distinct genetic variability and instability of the influenza virus genome enable the creation of a large number of different subtypes of avian influenza virus with altered characteristics in terms of antigenic properties, pathogenicity and host specificity. In 2014, a novel reassortant subtype of highly pathogenic avian influenza H5N8 was detected for the first time on the European continent. In the Republic of Serbia, the first outbreak of avian influenza subtype H5N8 was recorded at the end of 2016. The highest mortality rate during this outbreak was detected in the mute swans (Cygnus Olor) and the virus was also diagnosed in a small number of domestic hens in rural households. The aim of this PhD thesis was a comparative analysis of macroscopic and microscopic lesions, distribution and virus tropism as well as the determination of the inflammatory cell immunophenotyping in the organs of two different bird species naturally infected with the avian influenza virus subtype H5N8. The carcasses of 15 mute swans and 15 hens that died during the avian influenza outbreak were examined. In addition, the aim of this research was the identification and molecular characterization of the avian influenza virus detected in Serbia during the winter 2016-2017. The real-time RT-PCR method was performed for detection of H5N8 HPAI virus presence in materials originating from naturally infected birds. Pathohistological and immunohistochemical methods were used to determine the character of lesions, the distribution of viral antigen and immunophenotyping of inflammatory cells in the lung, heart, spleen, intestine, kidney, pancreas and brain tissue...

Jezik

srpski

Datum

2019

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-ND 2.0 AT - Creative Commons Autorstvo - Nekomercijalno - Bez prerada 2.0 Austria License.

http://creativecommons.org/licenses/by-nc-nd/2.0/at/legalcode

Predmet

OSNO - Opšta sistematizacija naučnih oblasti, Veterinarska patologija. Bolesti domaćih životinja

avijarna influenca, H5N8, labudovi, kokoške, patomorfološke promene, distribucija antigena

619:616.921.5

OSNO - Opšta sistematizacija naučnih oblasti, Veterinarska patologija. Bolesti domaćih životinja

avian influenza, H5N8, mute swans, hens, pathomorphological lesions, antigen distribution